Chymotryptic Digest of Papain

Chymotryptic digest of papain. III. Amino acid sequence of six peptides.

Dinitrophenylation-The amino-terminal residue was determined on. a sample of the peptide before desalting. Approximately 1 pmole of the peptide was submitted to dinitrophenylation for 6 hours with the reaction mixture maintained at pH 8.5 with the aid of a pH stat. The solution was evaporated to dryness and triturated with dry ethyl acetate. Estraction was continued until all the yellow color w...

Tryptic digest of papain. I. Isolation and sequence of some peptides from the oxidized protein.

Investigations are in progress in this laboratory to elucidate the amino acid sequence of the proteolytic enzyme, papain (2). Results have already been presented on the isolation and sequence of some peptides obtained after chymotryptic digestion of oxidized papain (3-5). In the present paper, we report the isolation, composition, and sequence of some peptides produced by tryptic digestion of o...

A New Vegetable Culture Medium Made from the Papain Digest of Mung Dal (Phaseolus Mungo), Green Variety

Primary Structure of the Calcium Ion-Transporting Adenosine Triphosphatase of Rabbit Skeletal Sarcoplasmic Reticulum SOLUBLE PEPTIDES FROM THE a-CHYMOTRYPTIC DIGEST OF THE CARBOXYMETHYLATED PROTEIN

The isolation of the soluble peptides from the chymotryptic digest of the calciumtransporting ATPase of rabbit skeletal sarcoplasmic reticulum is described. These peptides were partially sequenced and the information obtained was used to align tryptic peptides of the protein and to confirm sequences within the tryptic peptides. Details of the isolation of some peptides and the amino acid analys...

Chemical and Enzymic Studies on the Amino-terminal Sequence of Papain. a Reinvestigation.

Peptides derived from chymotryptic and tryptic digests of papain are under investigation, and the sequences of a number of these peptides have been reported earlier (1). The recognition of the NHz-terminal peptide of the protein from each digest is based on a knowledge of the NHz-terminal sequence of the intact protein. Thompson (2) established the first 3 residues as Ileu-Pro-Glu from his stud...